Product Name :
GRP94 polyclonal antibody Background :
Heat shock protein (HSP) molecular chaperones are environmental stress-inducible gene products. The human HSP 90 family includes 17 genes that fall into four classes: HSP90AA, HSP90AB, HSP90B and TRAP. HSP 90 family members guide the normal folding, intracellular disposition and proteolytic turnover of many key regulators of cell growth, differentiation and survival. HSP 90α, also designated HSP90A, HSP 86 and LPS-associated protein 2 (LAP2), is a cytosolic enhancer of inducible nitric-oxide synthase (iNOS), with chaperone activity that is important for the transcriptional activity of p53. HSP 90β, also designated HSP90B, HSP 84 and HSPC2, is a cytosolic protein that participates in signaling pathways with PKC e to protect cells from external damage, particularly in heat shock-mediated events. GRP 94, also known as tumor rejection antigen 1 (TRA1), ECGP and GP96, localizes to the ER, is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated in human endothelial cells in response to hypoxia by HIF-1. TRAP1 (TNF receptor-associated protein 1), also designated HSP 75) is a mitochondrial matrix component that plays a role in the induction of apoptosis in response to reactive oxygen species. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
GRP94 polyclonal antibody detects endogenous levels of GRP94 protein. Immunogen :
Peptide. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot analysis of GRP94 on different cell lysate using anti-GRP94 antibody at 1/1,000 dilution. Positive control： Lane1: HepG2 Lane2: NIH-3T3 Lane3: PC-12
• 
  ICC staining GRP94 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---