Product Name :
Ikaros polyclonal antibody Background :
Ikaros family members, including Ikaros and Helios, are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Similarly, Helios expression is detected primarily in T cells and in the earliest embryonic hemopoietic precursors and in adult stem cells. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and lL-2 mediated signaling events. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
Ikaros polyclonal antibody detects endogenous levels of Ikaros protein. Immunogen :
Recombinant protein Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot analysis of Ikaros on human thymus tissue lysate using anti-Ikaros antibody at 1/1,000 dilution.
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  Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Ikaros antibody. Counter stained with hematoxylin.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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