Product Name :
IQGAP1 polyclonal antibody Background :
IQGAP1, for IQ motif containing GTPase activating protein, is a Ras GAP-related, Actin-binding protein that interacts with the small GTPases Cdc42 and Rac1. The C-terminus of IQGAP1 is essential for interacting with Cdc42 and, in addition, IQGAP1 contains a WW domain and a predicted N-terminal coiled-coil region, which may be involved in IQGAP dimerization. Expression of IQGAP1 is highest in placenta, lung and kidney, where it co-localizes with Cdc42 to the cytoskeleton and assists with Cdc42 in mediating the regulation of cell proliferation, polarity and cell morphology. IQGAP1 regulates cadherin-mediated cell adhesion via binding to E-cadherin, b-catenin and a-catenin. This association induces the accumulation of these proteins at the site of cell-cell contact. IQGAP1 is negatively regulated by calmodulin, which binds to IQGAP1 in a calcium-dependent manner and disrupts IQGAP1 from associating with Cdc42. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles. Specificity :
IQGAP1 polyclonal antibody detects endogenous levels of IQGAP1 protein. Immunogen :
Recombinant protein corresponding to N-terminal human IQGAP1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of IQGAP1 on different lysates using anti-IQGAP1 antibody at 1/1,000 dilution. Positive control: Lane 1: HUVEC Lane 2: A431 Lane 3: Mouse placenta
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  ICC staining IQGAP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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