Product Name :
LSD1 polyclonal antibody Background :
Histone methylation regulates chromatin structure and transcription and maintains an epigenetic state of the cell. Histone methylation is dynamically regulated by histone methylases and demethylases. Lysine-specific histone demethylase 1 (LSD1), also designated BHC110, is a flavin-dependent amine oxidase which catalyzes the removal of one or two methyl groups from the methyl-lysine-4 side chain of Histone H3. The LSD1 protein contains a SWIRM domain, a FAD-binding motif and an amine oxidase domain. Association with CoREST, a SANT domain-containing corepressor, positively regulates LSD1. CoREST mediates the demethylation ability of LSD1 and protects it from proteasomal degradation in vivo. PHF21A (also designated BCH80), a PHD domain-containing protein, inhibits activity of LSD1/CoREST mediated de-methylation. The LSD1 protein also co-localizes with the androgen receptor in human prostate tumor cells and in unaffected prostate cells, stimulating androgen-receptor-dependent transcription. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
LSD1 polyclonal antibody detects endogenous levels of LSD1 protein. Immunogen :
Peptide Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of LSD1 on PC-12 and SiHa cell lysates using anti-LSD1 antibody at 1/1,000 dilution.
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  ICC staining LSD1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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