MAP1LC3A polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
MAP1LC3A polyclonal antibody Background :
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3β) and MAP-light chain 3 alpha (MAP-LC3α), both of which are mammalian homologs of yeast Apg8, are subunits that can associate with either MAP-1A or MAP-1B. While MAP-LC3β is essential for autophagy and is associated with autophagosome membranes after processing, MAP LC3α is involved in the formation of autophagosomal vacuoles and is localized to the intracytoplasmic membrane. MAP LC3α is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver and skeletal muscle, but are absent in thymus and peripheral blood leukocytes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
MAP1LC3A polyclonal antibody detects endogenous levels of MAP1LC3A protein. Immunogen :
recombinant protein Conjugate :
Unconjugated Modification :
Unmodification
MAP1LC3A polyclonal antibody Background :
Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3β) and MAP-light chain 3 alpha (MAP-LC3α), both of which are mammalian homologs of yeast Apg8, are subunits that can associate with either MAP-1A or MAP-1B. While MAP-LC3β is essential for autophagy and is associated with autophagosome membranes after processing, MAP LC3α is involved in the formation of autophagosomal vacuoles and is localized to the intracytoplasmic membrane. MAP LC3α is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver and skeletal muscle, but are absent in thymus and peripheral blood leukocytes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
MAP1LC3A polyclonal antibody detects endogenous levels of MAP1LC3A protein. Immunogen :
recombinant protein Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot analysis of MAP1LC3A on mouse brain lysates using anti-MAP1LC3A antibody at 1/1,000 dilution. -
ICC staining MAP1LC3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.