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MMP-1 polyclonal antibody BS90866
  • Western blot analysis of MMP1 on different cell lysates using anti-MMP1 antibody at 1/1000 dilution. Positive control: Lane 1: SKBR3 Lane 2: Raji Lane 3: A431 Lane 4: A549
  • ICC staining MMP1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product NameMMP-1 polyclonal antibody
Catalog No.BS90866
Swiss-ProtP03956
Host Rabbit
ReactivityHuman
ApplicationsWB ICC IHC
Application_allWB:1:1,000 ICC:1:200 IHC:1:200
BiowMW54 kDa
Alternative NameMMP-1, 27 kDa interstitial collagenase, CLG, CLGN, collagenase, fibroblast, collagenase, interstitial, Fibroblast collagenase, Interstitial collagenase, Matrix metallopeptidase 1 (interstitial collagenase), Matrix metalloprotease 1, Matrix Metalloproteinase 1, Matrix metalloproteinase-1, MMP 1, MMP-1, MMP1, MMP1, OTTHUMP00000045866,
Purification&PurityPeptide affinity purified
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $258
100ul $398
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Product Name :
MMP-1 polyclonal antibody
Background :
MMP-1 has an archetypal structure consisting of a pre-domain, a pro-domain, a catalytic domain, a linker region and a hemopexin-like domain. Two main nomenclatures for the primary structure are currently in use, the original one from which the first amino-acid starts with the signalling peptide and a second one where the first amino-acid starts counting from the prodomain (proenzyme nomenclature). MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Specifically, MMP-1 breaks down the interstitial collagens, types I, II, and III. Mechanical force may increase the expression of MMP1 in human periodontal ligament cells.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity :
MMP-1 polyclonal antibody detects endogenous levels of MMP-1 protein.
Immunogen :
peptide
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of MMP1 on different cell lysates using anti-MMP1 antibody at 1/1000 dilution. Positive control: Lane 1: SKBR3 Lane 2: Raji Lane 3: A431 Lane 4: A549
  • ICC staining MMP1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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