Product Name :
Orai3 polyclonal antibody Background :
ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3) is a Protein Coding gene. Among its related pathways are Calcium signaling pathway. Gene Ontology (GO) annotations related to this gene include store-operated calcium channel activity. An important paralog of this gene is ORAI2. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles. Specificity :
Orai3 polyclonal antibody detects endogenous levels of Orai3 protein. Immunogen :
Synthetic peptide within human Orai-3 aa 10-50. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of Orai3 using anti-Orai3 antibody at 1/500 dilution. Lane 1 : Raji cell lysates Lane 2 : Raji cell lysates(anti-Orai3 antibody+ immunizing peptide)
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  ICC staining Orai3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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