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PDHB polyclonal antibody BS91041
  • Western blot analysis of Pyruvate Dehydrogenase E1 beta subunit on HepG2 (1) and Hela (2) cell lysate using anti-Pyruvate Dehydrogenase E1 beta subunit antibody at 1/1,000 dilution.
  • Western blot analysis of Pyruvate Dehydrogenase E1 beta subunit on mouse kidney tissue (1) and PC-12 (2) cell lysate using anti-Pyruvate Dehydrogenase E1 beta subunit antibody at 1/1,000 dilution.
Product NamePDHB polyclonal antibody
Catalog No.BS91041
Swiss-ProtP11177(Human) Q9D051(Mouse) P49432(Rat)
Host Rabbit
ReactivityHuman, Mouse, Rat
ApplicationsWB IHC IP FC
Application_allWB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100
BiowMW
Alternative NamePDHE1-B, PDHB, PHE1B, DKFZp564K0164, mitochondrial, ODPB, pdhB, PDHBD, PDHE1 B, PDHE1-B, PHE1B, Pyruvate dehydrogenase (lipoamide) beta, Pyruvate dehydrogenase E1 beta polypeptide, Pyruvate dehydrogenase E1 component subunit beta, Pyruvate dehydrogenase E1 component subunit beta mitochondrial,
Purification&PurityProA affinity purified
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $258
100ul $398
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Product Name :
PDHB polyclonal antibody
Background :
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency). PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity :
PDHB polyclonal antibody detects endogenous levels of PDHB protein.
Immunogen :
Recombinant protein
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of Pyruvate Dehydrogenase E1 beta subunit on HepG2 (1) and Hela (2) cell lysate using anti-Pyruvate Dehydrogenase E1 beta subunit antibody at 1/1,000 dilution.
  • Western blot analysis of Pyruvate Dehydrogenase E1 beta subunit on mouse kidney tissue (1) and PC-12 (2) cell lysate using anti-Pyruvate Dehydrogenase E1 beta subunit antibody at 1/1,000 dilution.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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