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PU.1/Spi1 polyclonal antibody BS91136
  • Western blot analysis of PU.1/Spi1 on THP-1 cell using anti-PU.1/Spi1 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PU.1/Spi1 antibody. Counter stained with hematoxylin.
Product NamePU.1/Spi1 polyclonal antibody
Catalog No.BS91136
Swiss-ProtP17947(Human)
Host Rabbit
ReactivityHuman
ApplicationsWB IHC FC
Application_allWB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100
BiowMW31 kDa
Alternative NameSPI1, transcription factor spi1, 31 kDa Transforming Protein, 31 kDa-transforming protein, cb1086, Hematopoietic transcription factor PU.1, OF, oncogene spi1, PU.1, SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of, SFPI1, si:by184l24.2, SPI 1, SPI 1 proto oncogene, SPI A, Spi1, SPI1, Spleen focus forming virus (SFFV) proviral integration oncogene spi1, Spleen focus forming virus proviral integration oncogene spi1, Transcription factor PU.1,
Purification&PurityProA affinity purified
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $258
100ul $398
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Product Name :
PU.1/Spi1 polyclonal antibody
Background :
The Ets transcription factor family (Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF) are DNA-binding proteins that influence lymphoid development and activity. The Ets family monomeric proteins bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. PU.1 (Spi-1/Spi-A), Spi-B and Spi-C are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to the non-canonical AGAA sites. PU.1 transactivates a large number of B cell genes, such as those encoding CD72, CD20 and Btk, and Spi-B enhances expression of many of these same target genes. PU.1 is expressed in a wide variety of hematopoetic cells, including B cells, early T-cells, megakaryocytes, granulocytes, mast cells, immature erythrocytes and myeloid cells. Alternatively, Spi-B expression is limited to B cells and immature T cells, where expression accumulates through T-lineage commitment and then is dramatically absent following the beta-selection checkpoint.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity :
PU.1/Spi1 polyclonal antibody detects endogenous levels of PU.1/Spi1 protein.
Immunogen :
Recombinant protein
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of PU.1/Spi1 on THP-1 cell using anti-PU.1/Spi1 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PU.1/Spi1 antibody. Counter stained with hematoxylin.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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