Product Name :
RBX1 polyclonal antibody Background :
Rbx1 (also designated ROC1 and Hrt1) and the closely related protein Rbx2 (also designated ROC2) are RING finger containing homologs of the yeast protein APC11, a member of the anaphase-promoting complex (APC). Rbx1 was shown to be a component of the von Hippel-Lindau (VHL) transcription elongation complex, which includes VHL, Elongin B, Elongin C and Cullin-2. Rbx1 interacts with Cullin-1 in the SCF (Skp1-Cdc53-F-box protein) ubiquitin ligase complex (2-4). Rbx1 functions as a common subunit of SCF complexes required for ubiquination of various proteins including yeast G1 cyclins, IkBa and b-catenin (2-4). Rbx1 was shown to enhance the ubiquitin ligase activity of the VHL/Cullin-2 complex, and of the SCF/Cullin-1 complex. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
RBX1 polyclonal antibody detects endogenous levels of RBX1 protein. Immunogen :
Recombinant protein Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of RBX1 on HepG2 cell lysates using anti-RBX1 antibody at 1/500 dilution.
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  ICC staining RBX1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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