Product Name :
Src polyclonal antibody Background :
The major translational products of the Src gene family are membrane-associated tyrosine protein kinases that lack transmembrane and external amino acid sequences. By virtue of their common structural motifs, the Src family is composed of nine members in vertebrates, including c-Src, c-Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck and Blk. Src family kinases, which contain an amino-terminal cell membrane anchor followed by SH3 and SH2 domains, transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src family members are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. Different subcellular locations of Src family kinases may be important for the regulation of specific cellular processes, such as mitogenesis, cytoskeletal organization and membrane trafficking. c-Src (also designated pp60Src, Src p60 and proto-oncogene tyrosine protein kinase Src) is expressed in a broad range of tissue and cell types, although the highest levels of c-Src are detected in neuronal tissues and platelets. c-Src may play a role in events associated with both neuronal differentiation and maintenance of mature neuronal cell functions. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
Src polyclonal antibody detects endogenous levels of Src protein. Immunogen :
recombinant protein Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of Src on A431 cell lysates using anti-Src antibody at 1/1,000 dilution.
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  ICC staining Src in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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