Product Name :
CYP4F2 (M66) polyclonal antibody Background :
CYP4F isoforms are involved in the oxidation of important cellular mediators, such as leukotriene B4 (LTB4) and prostaglandins. CYP4F2 catalyzes ω-hydroxylation of LTB4 to a less potent proinflammatory eicosanoid, 20-OH-LTB4, as well as arachidonic acid. CYP4F2 is expressed in liver and kidney. Its expression is repressed by peroxisomal proliferators and induced by retinoic acid. X-linked adrenoleukodystrophy (X-ALD) is a severe neurodegenerative disorder biochemically characterized by elevated levels of very long-chain fatty acids (VLCFA). CYP4F2 participates in the ω-hydroxylation of VLCFAs, which may provide an alternative mode of treatment for X-ALD patients. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CYP4F2 (M66) polyclonal antibody detects endogenous levels of CYP4F2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 36-90 of Human CYP4F2. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot (WB) analysis of CYP4F2 polyclonal antibody at 1:1000 dilution Lane1:HEPG2 whole cell lysate(55ug) Lane2:LO2 whole cell lysate(38ug) Lane3:The liver tissue lysate of Rat(41ug) Lane4:The liver tissue lysate of Mouse(38ug)
• 
  Immunohistochemistry (IHC) analyzes of CYP4F2 (M66) pAb in paraffin-embedded human liver cancer tissue.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---