Product Name :
Ube2N polyclonal antibody Background :
Ube2N, a member of the E2 Ubiquitin-conjugating enzyme family, completes the second step of ubiquitination reactions, essentially targeting proteins for proteasomic degradation. When proteins are modified with ubiquitin in an important cellular mechanism targeting abnormal or short lived proteins, ubiquitin, a short protein of 76 amino acids, attaches to a lysine residue resting on the target protein. Multiple cycles of ubiquitination create a polyubiquitin chain that the proteasome recognizes and subsequently triggers the ATP-dependent unfolding of the target protein. This allows protoeolytic degradation of the target protein. These degradation products are highly expressed in heart and skeletal muscles. Ube2N moderates the transcriptional activation of target genes, affects the progress of cell differentiation and aging, and also influences the DNA repair pathway, further adding to the survival of cells after DNA damage. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles. Specificity :
Ube2N polyclonal antibody detects endogenous levels of Ube2N protein. Immunogen :
Recombinant protein within human Ube2N aa 1-150. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of Ube2N on different lysates using anti-Ube2N antibody at 1/1,000 dilution. Positive control: Lane 1: Daudi Lane 2: SH-SY-5Y Lane 3: Mouse spleen Lane 4: Rat spleen

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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