Product Name :
UQCRC2 polyclonal antibody Background :
Cytochrome c is a well characterized, mobile electron transport protein that is essential to energy conversion in all aerobic organisms. Cytochrome b associates with cytochrome c subunit 1 and the Rieske protein to form complex III (also designated cytochrome bc1 complex), which is involved in cellular respiration. Ubiquinol cytochrome c reductase (UQCRFS1), also referred to as Rieske iron-sulfur protein, represents an important subunit of complex III of the mitochondrial respiratory chain that transfers electrons from ubiquinol to cytochrome c. The UQCRFS1 complex is made up of 3 respiratory subunits (cytochrome b, cytochrome c1, Rieske protein), 2 core proteins, and 6 low-molecular weight proteins. Ubiquinol cytochrome-c reductase complex core protein 2 (UQCRC2) represents one of the core proteins of UQCRFS1, and it is required for the assembly of the complex. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles. Specificity :
UQCRC2 polyclonal antibody detects endogenous levels of UQCRC2 protein. Immunogen :
Recombinant protein within human UQCRC2 aa 100-350. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of UQCRC2 on rat heart tissue lysate using anti-UQCRC2 antibody at 1/5,000 dilution.
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  ICC staining UQCRC2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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