Product Name :
DDX19B (V38) polyclonal antibody Background :
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. DEAD box proteins contain two conserved RecA-like domains that adopt different open structures in the absence of nucleic acid and closed structure when bound to RNA and ATP. They also exhibit RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. DEAD box RNA helicase DEAD5 (Dbp5), also known as DEAD box protein 19B (DDX19B), is a 479 amino acid protein belonging to the DEAD box family. Localized to the cytoplasm and nuclear envelope, Dbp5 participates in the export of mRNA from the nucleus to the cytoplasm. Dbp5 is activated by interactions mediated by Gle1 and is inhibited by Nup214. Two named isoforms of Dbp5 exist as a result of alternative splicing events. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
DDX19B (V38) polyclonal antibody detects endogenous levels of DDX19B protein. Immunogen :
Synthetic peptide, corresponding to amino acids 6-52 of Human DDX19B. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of DDX19B (V38) pAb at 1:1000 dilution Lane1:L02 whole cell lysate(20ug) Lane2:A375 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(20ug)
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  Immunohistochemistry (IHC) analyzes of DDX19B (V38) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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