Product Name :
EGFL4 (R1274) polyclonal antibody Background :
EGFL4 (epidermal growth factor-like protein 4), also known as MEGF8 (multiple epidermal growth factor-like domains protein 8) or C19orf49, is a 2,845 amino acid single-pass type I membrane protein. EGFL4 contains 2 CUB domains, 5 EGF-like domains, 12 Kelch repeats, 4 laminin EGF-like domains and 7 PSI domains. Existing as two alternatively spliced isoforms, the gene encoding EGFL4 maps to human chromosome 19q13.2. Chromosome 19 consists of approximately 63 million bases, makes up over 2% of human genomic DNA, and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin superfamily members including the killer cell and leukocyte Ig-like receptors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
EGFL4 (R1274) polyclonal antibody detects endogenous levels of EGFL4 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 1245-1296 of Human EGFL4. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of EGFL4 (R1274) pAb at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:C6 whole cell lysate(40ug) Lane4:AML-12 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of EGFL4 (R1274) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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