Product Name :
NDUFB9 (E134) polyclonal antibody Background :
NDUFB9 (NADH dehydrogenase (ubiquinone) 1 β subcomplex, 9), also known as LYRM3 or B22, is a 179 amino acid protein that belongs to the complex I LYR family. Localized to the inner mitochondrial membrane, as well as to the matrix side of the peripheral membrane, NDUFB9 functions as an accessory subunit of the multi-subunit mitochondrial membrane respiratory chain NADH dehydrogenase complex I. Complex I plays an important role in the transfer of electrons from NADH to the respiratory chain, a process that is essential for cellular respiration. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NDUFB9 (E134) polyclonal antibody detects endogenous levels of NDUFB9 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 102-150 of Human NDUFB9. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of NDUFB9 (E134) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:EC9706 whole cell lysate(40ug) Lane4:Panc1 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of NDUFB9 (E134) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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