Product Name :
SRp46 (E72) polyclonal antibody Background :
The family of SR factors all contain one or more RNA recognition motifs (RRM) and an arginine/serine (RS)-rich domain, are essential for constitutive splicing and also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing-sites. An additional SR-associated protein, p32, tightly associates with SR factors and preferentially inhibits ASF/SF2 functioning as both a splicing enhancer and splicing repressor protein by preventing the stable interaction of ASF/SF2 and the RNA. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SRp46 (E72) polyclonal antibody detects endogenous levels of SRp46 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 50-100 of Human SRp46. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of SRp46 (E72) pAb at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:The Embryo tissue lysate of Mouse(40ug) Lane3:EC9706 whole cell lysate(20ug) Lane4:HEK293T whole cell lysate(10ug) Lane5:A549 whole cell lysate(20ug)
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  Immunohistochemistry (IHC) analyzes of SRp46 (E72) pAb in paraffin-embedded human colon cancer tissue.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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