Product Name :
Plasminogen (Y233) polyclonal antibody Background :
Cleavage of the serine proteinase plasminogen to form plasmin is the central event in the dissolution of blood clots by the fibrinolytic system. Within the fibrinolytic cascade, the serine proteinases urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) activate the proenzyme plasminogen by cleaving plasminogen to form the fibrinolytically active enzyme plasmin. The enzyme plasmin consists of a heavy chain of 561 amino acids, which originates from the N-terminus of plasminogen, and a light chain of 230 amino acid residues, which is derived from the C-terminus of plasminogen. Plasmin is a proangiogenic proteinase that is capable of degrading a variety of extracellular matrix proteins and that facilitates endothelial cell migration and angiogenesis. In the presence of free sulfhydryl donors (FSD), plasmin undergoes auto-proteolysis and is converted to the enzyme angiostatin, which blocks angiogenesis and neovascularization and can inhibit the growth of primary and metastatic tumors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Plasminogen (Y233) polyclonal antibody detects endogenous levels of Plasminogen protein. Immunogen :
Synthetic peptide, corresponding to amino acids 201-250 of Human Plasminogen. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot (WB) analysis of Plasminogen (Y233) pAb at 1:500 dilution Lane1:L02 whole cell lysate(10ug) Lane2:The Testis tissue lysate of Mouse(40ug) Lane3:The Testis tissue lysate of Rat(40ug) Lane4:K562 whole cell lysate(20ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---