Nrf3 (D530) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Nrf3 (D530) polyclonal antibody Background :
Nrf3 (nuclear factor (erythroid-derived 2)-like 3, NFE2L3, NF-E2-related factor 3, NRF3) is a transcription factor that influences placental gene expression and development. Nrf3 contains a Cap'n'collar (CNC)-type basic leucine zipper (bZIP) domain, and belongs to the CNC gene family. CNC genes play important roles in development, differentiation, oncogenesis, and stress signaling. Nrf3 protein is detectable in primary placental cytotrophoblasts. The human choriocarcinoma cell lines BeWo and JAR from trophoblastic tumors of the placenta express Nrf3 transcripts. Nrf3/MAFG heterodimers recognize nuclear factor-erythroid 2/Maf recognition element-type DNA-binding motifs. Keratinocyte growth factor (KGF)-regulates Nrf3-dependent gene expression during cutaneous wound repair. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Nrf3 (D530) polyclonal antibody detects endogenous levels of Nrf3 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 500-520 of Human Nrf3. Conjugate :
Unconjugated Modification :
Unmodification
Nrf3 (D530) polyclonal antibody Background :
Nrf3 (nuclear factor (erythroid-derived 2)-like 3, NFE2L3, NF-E2-related factor 3, NRF3) is a transcription factor that influences placental gene expression and development. Nrf3 contains a Cap'n'collar (CNC)-type basic leucine zipper (bZIP) domain, and belongs to the CNC gene family. CNC genes play important roles in development, differentiation, oncogenesis, and stress signaling. Nrf3 protein is detectable in primary placental cytotrophoblasts. The human choriocarcinoma cell lines BeWo and JAR from trophoblastic tumors of the placenta express Nrf3 transcripts. Nrf3/MAFG heterodimers recognize nuclear factor-erythroid 2/Maf recognition element-type DNA-binding motifs. Keratinocyte growth factor (KGF)-regulates Nrf3-dependent gene expression during cutaneous wound repair. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Nrf3 (D530) polyclonal antibody detects endogenous levels of Nrf3 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 500-520 of Human Nrf3. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Nrf3 (D530) pAb at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:The Uterus tissue lysate of Rat(40ug) Lane3:A375 whole cell lysate(40ug) Lane4:PC3 whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.