Product Name :
ADAM32 (N530) polyclonal antibody Background :
ADAM (disintegrin and metalloproteinase domain) proteins, also known as MDC (metalloproteinase, disintegrin and cysteine-rich domain) proteins or cellular disintegrins, are a family of proteins that are expressed in numerous tissues. ADAMs are membrane-anchored, glycosylated, Zn2+ dependent proteases that catalyze proteolysis, adhesion, fusion and intracellular signaling. The ADAM family consists of more than 30 different members with many diverse functions. ADAM32 is expressed predominantly in the testis on the sperm surface. The ADAM32 precursor originates in the testis and is processed during epididymal maturation. ADAM32 may play a role in sperm-egg adhesion or sperm development. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ADAM32 (N530) polyclonal antibody detects endogenous levels of ADAM32 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 500-560 of Human ADAM32. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of ADAM32 (N530) polyclonal antibody at 1:500 dilution Lane1:BV2 whole cell lysate(40ug) Lane2:The Testis tissue lysate of Rat(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:Hela whole cell lysate(20ug) Lane5:U-87MG whole cell lysate(30ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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