Product Name :
Cleaved-Endostatin (H1572) polyclonal antibody Background :
Collagen type XV (COL15 gene product) and XVIII (COL18 gene product) form the new subgroup MULTIPLEXIN, within the diverse family of collagens, which contains 19 distinct types of collagens found in vertebrates. Both Collagen Type XV and Type XVIII are characterized by extensive interruptions in their collagenous sequences. Members of the MULTIPLEXIN subgroup contain polypeptides with multiple triple-helical domains separated and flanked by nontriple-helical regions. Type XV is predominantly expressed in internal organs such as adrenal gland, kidney and pancreas. Type XVIII encodes 2 different α1 chains, which have different signal peptides and variant N-terminal non-collagenous NC1 domains of 495 and 303 amino acids. The long variant NC1-434 Type XVIII mRNAs are prominently expressed in liver, while the variant NC1- 303 mRNAs are predominantly expressed in heart, kidney, placenta, prostate, ovary, skeletal muscle and small intestine. Endostatin is a fragment of the Cterminal domain NC1 of Collagen Type XV and Type XVIII that inhibits angiogenesis and tumor growth. Unlike endostatin-XVIII, endostatin-XV does not bind zinc or heparin, however both endostatins can inhibit chorioallantoic membrane angiogenesis induced by basic FGF or VEGF. Collagen Type XV and XVIII are also widely present in basement membrane zones, suggesting their roles in basement membrane-stromal interactions and in-volvement with angiogenic and pathological processes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cleaved-Endostatin (H1572) polyclonal antibody detects endogenous levels of Cleaved-Endostatin protein. Immunogen :
Synthetic peptide, corresponding to amino acids 1572-1754 of Human Cleaved-Endostatin. Conjugate :
Unconjugated Modification :
Cleaved

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  Western blot (WB) analysis of Cleaved-Endostatin (H1572) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:L02 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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