Product Name :
PDGFRβ (phospho Y740) polyclonal antibody Background :
Platelet Derived Growth Factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PDGF-AA, PDGF-AB, and PDGF-BB. These three isoforms bind with different affinities to two receptor types, a and b, which are endowed with protein tyrosine kinase domains and undergo either homo- or heterodimerization as a consequence of ligand binding. Ligand stimulation of PDGFR-b leads to autophosphorylation at Tyr-857, which is the major autophosphorylation site, and Tyr-751, which is the major in vitro phosphorylation site. Autophosphorylation of Tyr-751, which lies in the kinase insert region, is required for binding of phosphatidylinositol-3 kinase to the receptor. These auto-phosphorylation events largely contribute to signal transduction through the PDGF receptor. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
PDGFRβ (phospho Y740) polyclonal antibody detects endogenous levels of PDGFRβ protein only when phosphorylated at Y740. Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Tyr740 of human PDGF Receptor beta. Conjugate :
Unconjugated Modification :
Phosphorylation

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  ICC staining PDGF Receptor beta(phospho Y740) in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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  ICC staining PDGF Receptor beta(phospho Y740) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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