Product Name :
ZO1 Rabbit monoclonal antibody Background :
Tight junction protein ZO-1 is a protein that in humans is encoded by the TJP1 gene. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This antibody detects endogenous levels of ZO1 and does not cross-react with related proteins. Immunogen :
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to ZO1. Conjugate :
Unconjugated Modification :
Unmodification

• 
• 
  Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZO1 antibody. Counter stained with hematoxylin.
• 
  Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZO1 antibody. Counter stained with hematoxylin.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---