Product Name :
PI3K p55γ Rabbit monoclonal antibody Background :
Phosphatidylinositol 3-kinase is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3’ position. PI 3-kinase p55γ (PIK3R3) is comprised of a catalytic subunit and a regulatory subunit. The human p55γ protein is composed of a rare amino terminal region followed by a proline-rich motif and two Src homology 2 (SH2) domains. PI 3-kinase p55γ mRNAs are expressed in most human fetal and adult tissues; predominant expression is observed in the adult testis. Splice variant(s) of PI 3-kinase p55γ have been identified; one of which has a deletion of 36 amino acids at the amino terminus and another which has an insertion of 59 amino acids at position 256 between the SH2 domains. Research suggests that PI 3-kinase p55γ interacts with the IGFIR (Insulin-like growth factor-I receptor) and IR (Insulin receptor) and may be involved in PI 3-kinase activation by these receptors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This antibody detects endogenous levels of PI3K p55γ and does not cross-react with related proteins. Immunogen :
Recombinant antibody. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of PI3K p55γ Rabbit mAb at 1:1000 dilution Lane1:MCF7 whole cell lysate Lane2:Jurkat whole cell lysate Lane3:The testis tissue lysate of Mouse
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  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PI 3 Kinase p55 γ antibody. Counter stained with hematoxylin.
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  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PI 3 Kinase p55 γ antibody. Counter stained with hematoxylin.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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