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PHB Rabbit monoclonal antibody BS9857M
  • Western blot (WB) analysis of PHB Rabbit mAb at 1:1000 dilution Lane1:293 whole cell lysate Lane2:Jurkat whole cell lysate Lane3:The kidney tissue lysate of Mouse Lane4:HepG2 whole cell lysate
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PHB antibody. Counter stained with hematoxylin.
Product NamePHB Rabbit monoclonal antibody
Catalog No.BS9857M
Swiss-ProtP35232
Host Rabbit
ReactivityHuman,Mouse,Rat, Zebra fish
ApplicationsWB ICC IHC IP FC
Application_allWB: 1:1000-1:2000 ICC: 1:50-1:200 IHC: 1:200-1:500 FC: 1:50-1:100
BiowMW~ 30 kDa
Alternative NameProhibitin
Purification&PurityProtein A affinity purified
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $258
100ul $398
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Product Name :
PHB Rabbit monoclonal antibody
Background :
Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
This antibody detects endogenous levels of PHB and does not cross-react with related proteins.
Immunogen :
Recombinant antibody.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of PHB Rabbit mAb at 1:1000 dilution Lane1:293 whole cell lysate Lane2:Jurkat whole cell lysate Lane3:The kidney tissue lysate of Mouse Lane4:HepG2 whole cell lysate
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PHB antibody. Counter stained with hematoxylin.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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