Product Name :
Ubiquitin Rabbit monoclonal antibody Background :
Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This antibody detects ubiquitin, polyubiquitin and ubiquitinated proteins. Immunogen :
Recombinant antibody. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of Ubiquitin Rabbit mAb at 1:1000 dilution Lane1:The heart tissue lysate of Mouse
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  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Ubiquitin antibody. Counter stained with hematoxylin.
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  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Ubiquitin antibody. Counter stained with hematoxylin.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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