Product Name :
Smad3 (phospho-S423/S425) Rabbit monoclonal antibody Background :
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), have been implicated as downstream effectors of TGFß/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP2 and BMP4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFß and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFß signaling by interfering with TGF∫-mediated phosphorylation of other Smad family members. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This antibody detects endogenous levels of Smad3 only when phosphorylated at Ser423 and/or Ser425. Immunogen :
Recombinant antibody. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of Smad3 (phospho-S423/S425) Rabbit mAb at 1:1000 dilution Lane1:A431 whole cell lysate Lane2:A431 whole cell lysate treated with TGF β
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  Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Phospho-Smad3 (S423/S425) antibody. Counter stained with hematoxylin.
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  Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Phospho-Smad3 (S423/S425) antibody. Counter stained with hematoxylin.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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