Tet1 (C-term) polyclonal antibody 
Datasheet
COA
MSDS
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Product Name :
Tet1 (C-term) polyclonal antibody Background :
Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5- hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5- formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Tet1 (C-term) polyclonal antibody detects endogenous levels of Tet1 protein. Immunogen :
This mouse Tet1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1845-1879 amino acids from the C-terminal region of mouse Tet1. Conjugate :
Unconjugated Modification :
Unmodification
Tet1 (C-term) polyclonal antibody Background :
Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5- hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5- formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Tet1 (C-term) polyclonal antibody detects endogenous levels of Tet1 protein. Immunogen :
This mouse Tet1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1845-1879 amino acids from the C-terminal region of mouse Tet1. Conjugate :
Unconjugated Modification :
Unmodification
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Anti-Tet1 Antibody (C-term)at 1:2000 dilution + F9 whole cell lysates Lysates/proteins at 20 µg per lane.
TET1 inhibits EMT of ovarian cancer cells through activating Wnt/β-catenin signaling inhibitors DKK1 and SFRP2
PMCID: Pubmed No.:28851501
The Tet1 and histone methylation expression pattern in dairy goat testis
PMCID: Pubmed No.:25662202
A postpartum enriched environment rescues impaired cognition and oxidative markers in aged mice with gestational inflammation
PMCID: Pubmed No.:36409568
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.