Beclin 1 monoclonal antibody 
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Product Name :
Beclin 1 monoclonal antibody Background :
BECN1 (beclin 1) is a coiled-coil protein that has been implicated as an_x000D_ inhibitor of tumorigenesis. BECN1, which associates with Bcl-2, plays a_x000D_ significant role in autophagy. Autophagy is the degradation of cellular proteins_x000D_ in the lysosomes, and when this pathway is suppressed, cell growth is_x000D_ deregulated. Autophagy is controlled by the same signal transduction pathway_x000D_ that induces the phosphorylation of the ribosomal protein S6, and both_x000D_ are mediated via amino acids. BECN1 expression in various carcinoma cell_x000D_ lines, such as MCF7, is low, whereas it is ubiquitously expressed in normal_x000D_ breast tissue. In transfected MCF7 cells, BECN1 complements autophagocytosis_x000D_ and, subsequently, inhibits cellular proliferation. Additionally, BECN1_x000D_ shares structural similarity to the yeast autophagy gene product, APG6, and_x000D_ was one of the first mammalian proteins discovered to mediate autophagy._x000D_ Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This antibody detects endogenous levels of Beclin 1 and does not cross-react with related proteins Immunogen :
Recombinant full length Human Beclin 1. Conjugate :
Unconjugated Modification :
Unmodification
Beclin 1 monoclonal antibody Background :
BECN1 (beclin 1) is a coiled-coil protein that has been implicated as an_x000D_ inhibitor of tumorigenesis. BECN1, which associates with Bcl-2, plays a_x000D_ significant role in autophagy. Autophagy is the degradation of cellular proteins_x000D_ in the lysosomes, and when this pathway is suppressed, cell growth is_x000D_ deregulated. Autophagy is controlled by the same signal transduction pathway_x000D_ that induces the phosphorylation of the ribosomal protein S6, and both_x000D_ are mediated via amino acids. BECN1 expression in various carcinoma cell_x000D_ lines, such as MCF7, is low, whereas it is ubiquitously expressed in normal_x000D_ breast tissue. In transfected MCF7 cells, BECN1 complements autophagocytosis_x000D_ and, subsequently, inhibits cellular proliferation. Additionally, BECN1_x000D_ shares structural similarity to the yeast autophagy gene product, APG6, and_x000D_ was one of the first mammalian proteins discovered to mediate autophagy._x000D_ Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This antibody detects endogenous levels of Beclin 1 and does not cross-react with related proteins Immunogen :
Recombinant full length Human Beclin 1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot detection of BECN1 in Mouse brain, A431&Hela cell lysates using BECN1 antibody (1:1000 diluted) .
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.