Product Name :
CHD4 monoclonal antibody Background :
The product of this gene belongs to the SNF2/RAD54 helicase family.It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression.Patients with dermatomyositis develop antibodies against this protein.Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CHD4 mAb detects endogenous levels of CHD4 protein. Immunogen :
Recombinant full length Human CHD4. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot detection of CHD4 in K562, HelaNE, HL-60, MOLT-4, Jurkat & 293 cell lysates using CHD4 antibody (1:1000 diluted) .

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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