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Csk monoclonal antibody MB0131
  • Western blot detection of CSK antibody in C6 and Jurkat cell lysates using CSK antibody (1:1000 diluted) .
Product NameCsk monoclonal antibody
Catalog No.MB0131
Swiss-ProtP41240
Host Mouse
ReactivityHuman,Rat
ApplicationsWB ICC
Application_allWB: 1:1000 ICC: 1:50
BiowMWPredicted band size:50KDa Observed band size:50KDa
Alternative NameTyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL; CSK
Purification&PurityThe antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $208
100ul $358
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Product Name :
Csk monoclonal antibody
Background :
C terminal Src kinase (CSK) is a non receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C terminal tyrosine of Src family kinases thereby down regulating their kinase activity. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft associated protein PAG (CSK binding protein).
Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
This antibody detects endogenous levels of Csk and does not cross-react with related proteins.
Immunogen :
Recombinant full length Human Csk.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot detection of CSK antibody in C6 and Jurkat cell lysates using CSK antibody (1:1000 diluted) .
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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