PCNA monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
PCNA monoclonal antibody Background :
The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This antibody detects endogenous levels of PCNA and does not cross-react with related proteins Immunogen :
Recombinant full length Human PCNA. Conjugate :
Unconjugated Modification :
Unmodification
PCNA monoclonal antibody Background :
The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This antibody detects endogenous levels of PCNA and does not cross-react with related proteins Immunogen :
Recombinant full length Human PCNA. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot detection of PCNA antibody in Hela, 3T3, COS7, C6 and CHO-K1 cell lysates using PCNA antibody (1:1000 diluted) . -
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An EGFR gene of the Pacific oyster Crassostrea gigas functions in wound healing and promotes cell proliferation
PMCID: Pubmed No.:24469720
Human mammary microenvironment better regulates the biology of human breast cancer in humanized mouse model
PMCID: Pubmed No.:25572806
Human mammary microenvironment better regulates the biology of human breast cancer in humanized mouse model
PMCID: Pubmed No.:25572806
An EGFR gene of the Pacific oyster Crassostrea gigas functions in wound healing and promotes cell proliferation
PMCID: Pubmed No.:24469720
Human mammary microenvironment better regulates the biology of human breast cancer in humanized mouse model
PMCID: Pubmed No.:25572806
MiR-3571 modulates the proliferation and migration of vascular smooth muscle cells by targeting claudin 1
PMCID: Pubmed No.:35370461
Hesperidin Reversed Long-Term N-methyl-N-nitro-N-Nitroguanidine Exposure Induced EMT and Cell Proliferation by Activating Autophagy in Gastric Tissues of Rats
PMCID: Pubmed No.:36558440
Krüppel-like Factor 7 inhibits proliferation and migration of pulmonary smooth muscle cells via p21 activation
PMCID: Pubmed No.:36566916
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.