Product Name :
EGFR (3F12) monoclonal antibody Background :
The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Ligand binding promotes the internalization of EGFR via Clathrin-coated pits and its subsequent degradation in response to its intrinsic tyrosine kinase. EGFR is involved in organ morphogenesis and maintenance and repair of tissues, but upregulation of EGFR is associated with tumor progression. The oncogenic effects of EGFR include initiation of DNA synthesis, enhanced cell growth, invasion and metastasis. Abrogation of EGFR results in cell cycle arrest, apoptosis or dedifferentiation of cancer cells, suggesting that EGFR may be an effective therapeutic target. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This antibody detects endogenous levels of EGFR and does not cross-react with related proteins. Immunogen :
Purified recombinant human EGFR protein fragments expressed in E.coli. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot detection of EGFR in HCC827,A549 and MDA-MB-468 cell lysates using EGFR mouse mAb(dilution 1:1000).
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  Immunohistochemical analysis of paraffin-embedded Colorectal cancer using EGF Receptor (3F12) mouse mAb (1/800 dilution). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
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  Immunohistochemical analysis of paraffin-embedded Colorectal cancer using EGF Receptor (3F12) mouse mAb (1/800 dilution). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
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  Immunohistochemical analysis of paraffin-embedded Colorectal cancer using EGF Receptor (3F12) mouse mAb (1/800 dilution). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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