Product Name :
AMPKβ1 (1A7) monoclonal antibody Background :
AMPKβ1 is a member of the SNF1 kinase family. It is a heterotrimeric protein comprising of an alpha (catalytic), beta (non-catalytic) and gamma (non-catalytic) subunits, 63, 38 and 38kDa respectively.AMPKβ1 regulates fatty acid and sterol synthesis by phosphorylation of acteyl-coA. It also regulates cholesterol synthesis via phosphorylation and in-activation of hydroxymethylglutaryl-CoA reductase.Two isoforms of AMPKβ1 have been found. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
This antibody detects endogenous levels of AMPKβ1 and does not cross-react with related proteins. Immunogen :
Purified recombinant human AMPKβ1 protein fragments expressed in E.coli. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot detection of AMPKβ1 in 3T3,Hela,PC-12,COS7 and MDA-MB-468 cell lysates using AMPKβ1 mouse mAb (1:1000 diluted).
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  Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPKβ1 mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
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  Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPKβ1 mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
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  Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPKβ1 mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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