T7-Tag Mouse monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
T7-Tag Mouse monoclonal antibody Background :
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors that is based on the very efficient T7 RNA polymerase expression system. Antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. Product :
Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
T7-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal T7- tagged proteins. Immunogen :
Synthetic peptide T7-Tag. Conjugate :
Unconjugated Modification :
Unmodification
T7-Tag Mouse monoclonal antibody Background :
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors that is based on the very efficient T7 RNA polymerase expression system. Antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. Product :
Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
T7-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal T7- tagged proteins. Immunogen :
Synthetic peptide T7-Tag. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of GST-T7 fusion protein, using T7-tag mouse monoclonal antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.