Product Name :
BHMT monoclonal antibody Background :
Betaine-homocysteine methyltransferase (BHMT) is a zinc-dependent cytosolic protein that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT is highly expressed in rat liver, and its expression is regulated by dietary methionine and choline. In humans, S-adenosylmethionine (SAM) down-regulates BHMT expression by inducing NFκB, which acts as a repressor for the BHMT gene. Lowered BHMT levels can lead to ER (endoplasmic reticulum) stress. Mutations in the gene encoding for BHMT may lead to hyperhomocysteinemia, a medical condition characterized by abnormally large amounts of homocysteine in the blood which may be a risk factor for cardiovascular and cerebrovascular diseases. Product :
Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles. Specificity :
BHMT monoclonal antibody detects endogenous levels of BHMT protein. Immunogen :
Recombinant protein. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot analysis of BHMT on human liver tissue and human kidney tissue lysate using anti-BHMT antibody at 1/1,000 dilution.
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  ICC staining BHMT (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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