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ERp72 monoclonal antibody MB9209
  • Western blot analysis of ERp72 on A431 cell lysate using anti-ERp72 antibody at 1/500 dilution.
  • ICC staining ERp72 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product NameERp72 monoclonal antibody
Catalog No.MB9209
Swiss-ProtP13667(Human) P08003(Mouse) P38659(Rat)
Host Mouse
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC ICC FC
Application_allWB:1:500-1:1,000 ICC:1:50 IHC:1:50-1:200 FC:1:50-1:100
BiowMW73 kDa
Alternative NameER protein 70, ERp70, ER protein 72, ERp-72, ERp72, PDIA4, Calcium binding protein intestinal related, Endoplasmic reticulum resident protein 70, Endoplasmic reticulum resident protein 72, EPR70, EPR72, ER protein 70, ER protein 72, ERP 70, ERp 72, ERp-72, ERp70, ERp72, PDIA 4, Pdia4, PDIA4, PDIR, Protein disulfide isomerase A4, Protein disulfide isomerase associated 4, Protein disulfide isomerase family A member 4, protein disulfide isomerase related protein (calcium-binding protein, intestinal-related), Protein disulfide isomerase related protein, Protein disulfide-isomerase A4, Protein ERp 72, Protein ERp72,
Purification&PurityProG purified.
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $258
100ul $398
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Product Name :
ERp72 monoclonal antibody
Background :
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly.
Product :
Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity :
ERp72 monoclonal antibody detects endogenous levels of ERp72 protein.
Immunogen :
Synthetic peptide within C-terminal human ERp72.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of ERp72 on A431 cell lysate using anti-ERp72 antibody at 1/500 dilution.
  • ICC staining ERp72 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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