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GST monoclonal antibody MB9256
  • Western blot analysis of GST on GST-tagged recombinant protein using anti-GST antibody at 1/5000 dilution.
Product NameGST monoclonal antibody
Catalog No.MB9256
Swiss-Prot
Host Mouse
Reactivity
ApplicationsWB ELISA
Application_allWB:1:5,000 ELISA:1:10,000-1:20,000
BiowMW26 kDa
Alternative Name, Glutathione S Transferase, Glutathione S-transferase class-mu 26 kDa isozyme, GST, Sj26 antigen, SjGST,
Purification&PurityProA affinity purified
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $258
100ul $398
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Product Name :
GST monoclonal antibody
Background :
The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest.
Product :
Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity :
GST monoclonal antibody detects endogenous levels of GST protein.
Immunogen :
recombinant protein
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of GST on GST-tagged recombinant protein using anti-GST antibody at 1/5000 dilution.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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