NRF2 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
NRF2 polyclonal antibody Background :
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Polyclonal Antibodies Immunogen :
Recombinant fusion protein of human NRF2(NP_006155.2). Conjugate :
Unconjugated Modification :
Unmodification
NRF2 polyclonal antibody Background :
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Polyclonal Antibodies Immunogen :
Recombinant fusion protein of human NRF2(NP_006155.2). Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of various lysates, using NRF2 antibody at ARC50393-02 dilution.HeLa cells were treated by MG132 at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s. -
Western blot analysis of various lysates, using NRF2 antibody at ARC50393-02 dilution.NIH3T3 cells were treated by MG132 at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s. -
Western blot analysis of various lysates, using NRF2 antibody at ARC50393-02 dilution.NIH3T3 cells were treated by MG132 at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s. -
Western blot analysis of various lysates, using NRF2 antibody at ARC50393-02 dilution.NIH3T3 cells were treated by MG132 at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
Aged red garlic extract reduces lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages and acute pulmonary inflammation through heme oxygenase-1 induction
PMCID: Pubmed No.:22353229
Drug resistance associates with activation of Nrf2 in MCF-7/DOX cells, and wogonin reverses it by down-regulating Nrf2-mediated cellular defense response
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SHPS-1 deficiency induces robust neuroprotection against experimental stroke by attenuating oxidative stress
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Olmesartan ameliorates urinary dysfunction in the spontaneously hypertensive rat via recovering bladder blood flow and decreasing oxidative stress
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Curcumin upregulates Nrf2 nuclear translocation and protects rat hepatic stellate cells against oxidative stress
PMCID: Pubmed No.:26676408
Curcumin upregulates Nrf2 nuclear translocation and protects rat hepatic stellate cells against oxidative stress
PMCID: Pubmed No.:26676408
Apigenin, a modulator of PPARγ, attenuates HFD-induced NAFLD by regulating hepatocyte lipid metabolism and oxidative stress via Nrf2 activation
PMCID: Pubmed No.:28414138
Reduction of oxidative damages induced by titanium dioxide nanoparticles correlates with induction of the Nrf2 pathway by GSPE supplementation in mice
PMCID: Pubmed No.:28780322
Curcumin upregulates Nrf2 nuclear translocation and protects rat hepatic stellate cells against oxidative stress
PMCID: Pubmed No.:26676408
Knockdown of a novel lincRNA AATBC suppresses proliferation and induces apoptosis in bladder cancer
PMCID: Pubmed No.:25473900
Administration of Donor-Derived Nonexpanded Adipose Stromal Vascular Fraction Attenuates Ischemia-Reperfusion Injury in Donation After Cardiac Death Rat Renal Transplantation
PMCID: Pubmed No.:34266654
Moringa oleifera mitigates ethanol-induced oxidative stress, fatty degeneration and hepatic steatosis by promoting Nrf2 in mice
PMCID: Pubmed No.:35358929
Ubiquitin-specific protease 7 regulates myocardial ischemia/reperfusion injury by stabilizing Keap1
PMCID: Pubmed No.:35710902
Therapeutic propensity of ginsenosides Rg1 and Rg3 in rhabdomyolysis-induced acute kidney injury and renohepatic crosstalk in rats
PMCID: Pubmed No.:36580761
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.