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[KO Validated] Caspase-3 polyclonal antibody BS6428
  • Western blot analysis of extracts of various cell lines, using Caspase-3 Rabbit pAb at 1:1000 dilution.Jurkat cells were treated by Etoposide at 37℃ for 5 hours.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% BSA.
    Detection: ECL Basic Kit .
    Exposure time: 3min.
  • Western blot analysis of extracts from normal and Caspase-3 knockout 293T cells, using Caspase-3 antibody at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit .
    Exposure time: 5s.
  • Western blot analysis of extracts from normal and Caspase-3 knockout 293T cells, using Caspase-3 antibody at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit .
    Exposure time: 5s.
  • Western blot analysis of extracts from normal and Caspase-3 knockout 293T cells, using Caspase-3 antibody at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit .
    Exposure time: 5s.
Product Name[KO Validated] Caspase-3 polyclonal antibody
Catalog No.BS6428
Swiss-ProtP42574
Host Rabbit
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF/ICC, IP
Application_allWB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:100|IP,1:50 - 1:100
BiowMW17kDa/35kDa
Alternative NameCPP32;CPP32B;SCA-1;Active Caspase 3;CASP3;active Caspase-3;Caspase 3;Caspase-3 p12;caspase-3
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50 ul $258
100 ul $398
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Product Name :
[KO Validated] Caspase-3 polyclonal antibody
Background :
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Polyclonal Antibodies
Immunogen :
Recombinant fusion protein of human Caspase-3(NP_004337.2).
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of extracts of various cell lines, using Caspase-3 Rabbit pAb at 1:1000 dilution.Jurkat cells were treated by Etoposide at 37℃ for 5 hours.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% BSA.
    Detection: ECL Basic Kit .
    Exposure time: 3min.
  • Western blot analysis of extracts from normal and Caspase-3 knockout 293T cells, using Caspase-3 antibody at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit .
    Exposure time: 5s.
  • Western blot analysis of extracts from normal and Caspase-3 knockout 293T cells, using Caspase-3 antibody at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit .
    Exposure time: 5s.
  • Western blot analysis of extracts from normal and Caspase-3 knockout 293T cells, using Caspase-3 antibody at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit .
    Exposure time: 5s.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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