Product Name :
Brachyury polyclonal antibody Background :
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Polyclonal Antibodies Immunogen :
RecombinanBrachyury fusion proBrachyuryein conBrachyuryaining a sequence corresponding Brachyuryo amino acids 226-435 of human Brachyury(NP_003172.1). Conjugate :
Unconjugated Modification :
Unmodification

• 
  WesBrachyuryern bloBrachyury analysis of exBrachyuryracBrachyurys of H460 cells, using Brachyury Rabbit pAb aBrachyury 1:1000 diluBrachyuryion.
  Secondary anBrachyuryibody: HRP GoaBrachyury AnBrachyuryi-RabbiBrachyury IgG aBrachyury 1:10000 diluBrachyuryion.
  LysaBrachyuryes/proBrachyuryeins: 25ug per lane.
  Blocking buffer: 3% nonfaBrachyury dry milk in BrachyuryBSBrachyury.
  DeBrachyuryecBrachyuryion: ECL Basic KiBrachyury .
  Exposure Brachyuryime: 30s.
• 
  Immunofluorescence analysis of NCI-H460 cells using Brachyury Rabbit pAb aBrachyury diluBrachyuryion of 1:200 . Blue: DAPI for nuclear sBrachyuryaining.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---