CDKN1C/p57 Kip2 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
CDKN1C/p57 Kip2 polyclonal antibody Background :
This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Polyclonal Antibodies Immunogen :
Recombinant fusion protein of human CDKN1C/p57 Kip2(NP_000067.1). Conjugate :
Unconjugated Modification :
Unmodification
CDKN1C/p57 Kip2 polyclonal antibody Background :
This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Polyclonal Antibodies Immunogen :
Recombinant fusion protein of human CDKN1C/p57 Kip2(NP_000067.1). Conjugate :
Unconjugated Modification :
Unmodification
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Western blot analysis of extracts of various cell lines, using CDKN1C/p57 Kip2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation
PMCID: Pubmed No.:27039827
MiR-222 overexpression promotes proliferation of human hepatocellular carcinoma HepG2 cells by downregulating p27
PMCID: Pubmed No.:24955159
MicroRNA-221 is Required for Proliferation of Mouse Embryonic Stem Cells via P57 Targeting
PMCID: Pubmed No.:25086570
Repetitive Transcranial Magnetic Stimulation Promotes Neural Stem Cell Proliferation via the Regulation of MiR-25 in a Rat Model of Focal Cerebral Ischemia
PMCID: Pubmed No.:25302788
MicroRNA-222 Controls Human Pancreatic Cancer Cell Line Capan-2 Proliferation by P57 Targeting
PMCID: Pubmed No.:26535064
mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation
PMCID: Pubmed No.:27039827
MicroRNA-221 is Required for Proliferation of Mouse Embryonic Stem Cells via P57 Targeting
PMCID: Pubmed No.:25086570
Repetitive Transcranial Magnetic Stimulation Promotes Neural Stem Cell Proliferation via the Regulation of MiR-25 in a Rat Model of Focal Cerebral Ischemia
PMCID: Pubmed No.:25302788
MicroRNA-222 Controls Human Pancreatic Cancer Cell Line Capan-2 Proliferation by P57 Targeting
PMCID: Pubmed No.:26535064
mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation
PMCID: Pubmed No.:27039827
The differential susceptibilities of MCF-7 and MDA-MB-231 cells to the cytotoxic effects of curcumin are associated with the PI3K/Akt-SKP2-Cip/Kips pathway
PMCID: Pubmed No.:25530715
Overexpression of transcription factor FoxA2 in the developing skeleton causes an enlargement of the cartilage hypertrophic zone, but it does not trigger ectopic differentiation in immature chondrocytes
PMCID: Pubmed No.:35398294
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.