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Cystathionase/CTH polyclonal antibody BS7916
  • Western blot analysis of various lysates, using Cystathionase/CTH antibody at 1:570 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit .
    Exposure time: 30s.
  • Immunofluorescence analysis of HepG2 cells using Cystathionase/CTH Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using Cystathionase/CTH Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.
Product NameCystathionase/CTH polyclonal antibody
Catalog No.BS7916
Swiss-ProtP32929
Host Rabbit
ReactivityHuman, Mouse, Rat
ApplicationsWB, IF/ICC, IP
Application_allWB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200
BiowMW45KDa
Alternative NameCTH
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50 ul $208
100 ul $358
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Product Name :
Cystathionase/CTH polyclonal antibody
Background :
This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms.
Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Polyclonal Antibodies
Immunogen :
Recombinant fusion protein of human Cystathionase/Cystathionase/CTH(NP_001893.2).
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of various lysates, using Cystathionase/CTH antibody at 1:570 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit .
    Exposure time: 30s.
  • Immunofluorescence analysis of HepG2 cells using Cystathionase/CTH Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using Cystathionase/CTH Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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