ATGL/PNPLA2 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
ATGL/PNPLA2 polyclonal antibody Background :
This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Polyclonal Antibodies Immunogen :
Recombinant fusion protein of human PNPLA2(NP_065109.1). Conjugate :
Unconjugated Modification :
Unmodification
ATGL/PNPLA2 polyclonal antibody Background :
This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Polyclonal Antibodies Immunogen :
Recombinant fusion protein of human PNPLA2(NP_065109.1). Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot analysis of extracts of various cell lines, using ATGL/PNPLA2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s. -
Immunofluorescence analysis of U2OS cells using ATGL/PNPLA2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Corticotrophin-releasing factor participates in S1PR3-dependent cPLA2 expression and cell motility in vascular smooth muscle cells
PMCID: Pubmed No.:25869501
Maternal betaine supplementation attenuates glucocorticoid-induced hepatic lipid accumulation through epigenetic modification in adult offspring rats
PMCID: Pubmed No.:29331496
miR-103 promotes 3T3-L1 cell adipogenesis through AKT/mTOR signal pathway with its target being MEF2D
PMCID: Pubmed No.:25400071
Chronic dexamethasone exposure markedly decreased the hepatic triglyceride accumulation in growing goats
PMCID: Pubmed No.:29155266
Butyrate stimulates adipose lipolysis and mitochondrial oxidative phosphorylation through histone hyperacetylation‐associated β3‐adrenergic receptor activation in high‐fat diet‐induced obese mice
PMCID: Pubmed No.:28028849
Alpha-Lipoic Acid Alleviates Acute Inflammation and Promotes Lipid Mobilization During the Inflammatory Response in White Adipose Tissue of Mice
PMCID: Pubmed No.:27531126
Maternal butyrate supplementation induces insulin resistance associated with enhanced intramuscular fat deposition in the offspring
PMCID: Pubmed No.:28055958
ZAG alleviates HFD-induced insulin resistance accompanied with decreased lipid depot in skeletal muscle in mice
PMCID: Pubmed No.:30318474
Zinc-α2-glycoprotein promotes browning of white adipose tissue in cold-exposed male mice
PMCID: Pubmed No.:31790716
Adipokine zinc-α2-glycoprotein alleviates lipopolysaccharide-induced inflammatory responses through the β3-AR/PKA/CREB pathway
PMCID: Pubmed No.:31260855
Zinc-α2-glycoprotein promotes skeletal muscle lipid metabolism in cold-stressed mice
PMCID: Pubmed No.:32863292
m6A-Mediated PPARA Translational Suppression Contributes to Corticosterone-Induced Visceral Fat Deposition in Chickens
PMCID: Pubmed No.:36555401
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.