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MYLK3 polyclonal antibody BS8336
  • Immunofluorescence analysis of U2OS cells using MYLK3 antibody . Blue: DAPI for nuclear staining.
Product NameMYLK3 polyclonal antibody
Catalog No.BS8336
Swiss-ProtQ32MK0
Host Rabbit
ReactivityHuman, Mouse
ApplicationsWB, IF/ICC
Application_allWB,1:500 - 1:2000|IF/ICC,1:50 - 1:100
BiowMWRefer to Figures
Alternative NameMYLK3;MLCK;MLCK2;caMLCK
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50 ul $208
100 ul $358
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Product Name :
MYLK3 polyclonal antibody
Background :
Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 [PubMed 18202317]).[supplied by OMIM, Jul 2008]
Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Polyclonal Antibodies
Immunogen :
Recombinant fusion protein of human MYLK3(NP_872299.2).
Conjugate :
Unconjugated
Modification :
Unmodification
  • Immunofluorescence analysis of U2OS cells using MYLK3 antibody . Blue: DAPI for nuclear staining.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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