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PHIP polyclonal antibody BS8775
  • Western blot analysis of extracts of various cell lines, using PHIP antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PHIP antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PHIP antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PHIP antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product NamePHIP polyclonal antibody
Catalog No.BS8775
Swiss-ProtQ8WWQ0
Host Rabbit
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF/ICC
Application_allWB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:100
BiowMW250kDa
Alternative NamePHIP;BRWD2;DCAF14;WDR11;ndrp
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50 ul $208
100 ul $358
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Product Name :
PHIP polyclonal antibody
Background :
This gene encodes a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. The encoded protein may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.
Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Polyclonal Antibodies
Immunogen :
Recombinant fusion protein of human PHIP(NP_060404.3).
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of extracts of various cell lines, using PHIP antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PHIP antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PHIP antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PHIP antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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