Product Name :
PPARγ (Phospho-Ser112) polyclonal antibody Background :
Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator. PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Besides its role in mediating adipogenesis and lipid metabolism, PPARγ also modulates insulin sensitivity, cell proliferation and inflammation. PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PPARγ(Phospho-Ser112) polyclonal antibody detects endogenous levels of PPARγ protein only when phosphorylated at Ser112. Immunogen :
Synthetic phosphopeptide derived from human PPARγ around the phosphorylation site of Serine 112. Conjugate :
Unconjugated Modification :
Phosphorylated

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  Western blot (WB) analysis of PPARγ (Phospho-Ser112) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(30ug) Lane2:MCF-7 treated with λ-phosphatase whole cell lysate(30ug) Lane3:PC3 whole cell lysate(30ug) Lane4:PC3 treated with λ-phosphatase whole cell lysate(30ug) Lane5:CT-26 whole cell lysate(30ug) Lane6:PMVEC whole cell lysate(30ug)
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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