Product Name :
AKT (phospho-T308) polyclonal antibody Background :
AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-AKT (T308) polyclonal antibody detects endogenous levels of AKT1 only when phosphorylated at Thr308. This antibody also recognizes AKT2 and AKT3 when phosphorylated at the corresponding residues. Immunogen :
Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 308. Conjugate :
Unconjugated Modification :
Phosphorylated
AKT (phospho-T308) polyclonal antibody Background :
AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-AKT (T308) polyclonal antibody detects endogenous levels of AKT1 only when phosphorylated at Thr308. This antibody also recognizes AKT2 and AKT3 when phosphorylated at the corresponding residues. Immunogen :
Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 308. Conjugate :
Unconjugated Modification :
Phosphorylated
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Western blot analysis of AKT (Phospho-T308) expression in HeLa colchicine-treated (A), HL60 (B), NIH3T3 (C), SP2/0 colchicine-treated (D), PC12 colchicine-treated (E) whole cell lysates.
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Immunohistochemical analysis of AKT (Phospho-T308) staining in human lung cancer formalin fixed paraffin embedded tissue section.
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Effect of CIPC and intervention of Ca2+-regulated factors on CaN, cbl-b and p-AKT expression in neurons
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Highly frequent PIK3CA amplification is associated with poor prognosis in gastric cancer
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Puerarin attenuates endothelial insulin resistance through inhibition of inflammatory response in an IKKβ/IRS-1-dependent manner
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Glucose oxidase induces insulin resistance via influencing multiple targets in vitro and in vivo: The central role of oxidative stress
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Positive and negative regulation of insulin action by genistein in the endothelium
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Interferon regulatory factor 3 is a negative regulator of pathological cardiac hypertrophy
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Quercetin and quercetin-3-O-glucuronide are equally effective in ameliorating endothelial insulin resistance through inhibition of reactive oxygen species-associated inflammation
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The expression of chitinase 3-like 1: a novel prognostic predictor for hepatocellular carcinoma
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Mechanisms underlying protective effects of trimetazidine on endothelial progenitor cells biological functions against H2O2-induced injury: Involvement of antioxidation and Akt/eNOS signaling pathways
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Effect of 5-hydroxymethylfurfural derived from processed Cornus officinalis on the prevention of high glucose-induced oxidative stress in human umbilical vein endothelial cells and its mechanism
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miR-145 inhibits isoproterenol-induced cardiomyocyte hypertrophy by targeting the expression and localization of GATA6.
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Sprengerinin C exerts anti-tumorigenic effects in hepatocellular carcinoma via inhibition of proliferation and angiogenesis and induction of apoptosis
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A novel role of ribonuclease inhibitor in regulation of epithelial-to-mesenchymal transition and ILK signaling pathway in bladder cancer cells
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β-Catenin overexpression is associated with gefitinib resistance in non-small cell lung cancer cells
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Small interfering RNA targeting ILK inhibits metastasis in human tongue cancer cells through repression of epithelial-to-mesenchymal transition
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The Study of Traditional Chinese Medical Elongated-needle Therapy promoting Neurological recovery mechanism after Spinal Cord Injury in Rats
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Ameliorative effect of ampelopsin on LPS-induced acute phase response in piglets
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Folate deprivation induces cell cycle arrest at G0/G1 phase and apoptosis in hippocampal neuron cells through down-regulation of IGF-1 signaling pathway
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CXCR4 knockdown inhibits the growth and invasion of nasopharyngeal cancer stem cells
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Osteosarcoma cells induce differentiation of mesenchymal stem cells into cancer associated fibroblasts through Notch and Akt signaling pathway
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Lnc-MCEI mediated the chemosensitivity of esophageal squamous cell carcinoma via miR-6759-5p to competitively regulate IGF2
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DNA Hypomethylation of GR Promoters is Associated with GR Activation and BDNF/AKT/ERK1/2‐Induced Hippocampal Neurogenesis in Mice Derived From Folic‐Acid‐Supplemented Dams
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Bisphenol A suppresses proliferation and induces apoptosis in colonic epithelial cells through mitochondrial and MAPK/AKT pathways
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Traditional Chinese Medicine CFF‐1 induced cell growth inhibition, autophagy, and apoptosis via inhibiting EGFR‐related pathways in prostate cancer
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NAB2-STAT6 fusion protein mediates cell proliferation and oncogenic progression via EGR-1 regulation
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Dysfunction of insulin-AKT-UCP1 signalling inhibits transdifferentiation of human and mouse white preadipocytes into brown-like adipocytes
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LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.