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SDHA polyclonal antibody BS7220
  • Western blot analysis of SDHA expression in HepG2 (A), MCF7 (B), mouse liver (C), mouse stomach (D) whole cell lysates.
  • Immunohistochemical analysis of SDHA staining in human liver cancer formalin fixed paraffin embedded tissue section.
  • Immunohistochemical analysis of SDHA staining in human liver cancer formalin fixed paraffin embedded tissue section.
Product NameSDHA polyclonal antibody
Catalog No.BS7220
Swiss-ProtP31040
Host Rabbit
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF/ICC
Application_allWB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200
BiowMW~ 70 kDa
Alternative NameSDHA;CMD1GG;FP;PGL5;SDH1;SDH2;SDHF
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodified
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Size Price
50ul $208
100ul $358
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Product Name :
SDHA polyclonal antibody
Background :
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
SDHA polyclonal antibody detects endogenous levels of SDHA protein.
Immunogen :
Recombinant full length protein of human SDHA.
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of SDHA expression in HepG2 (A), MCF7 (B), mouse liver (C), mouse stomach (D) whole cell lysates.
  • Immunohistochemical analysis of SDHA staining in human liver cancer formalin fixed paraffin embedded tissue section.
  • Immunohistochemical analysis of SDHA staining in human liver cancer formalin fixed paraffin embedded tissue section.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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